Journal: iScience

Article Title: Naive and memory CD4 + T cell subsets can contribute to the generation of human Tfh cells

doi: 10.1016/j.isci.2021.103566

Figure Lengend Snippet: CyTOF antibody panel

Article Snippet: Pure anti-human CCR5 , Miltenyi Biotec , Cat# 130-122-313; RRID:AB_2801894.

Techniques:

Journal: iScience

Article Title: Naive and memory CD4 + T cell subsets can contribute to the generation of human Tfh cells

doi: 10.1016/j.isci.2021.103566

Figure Lengend Snippet: Selected genes involved in Tfh cell biology

Article Snippet: Pure anti-human CCR5 , Miltenyi Biotec , Cat# 130-122-313; RRID:AB_2801894.

Techniques: Activation Assay

Journal: iScience

Article Title: Naive and memory CD4 + T cell subsets can contribute to the generation of human Tfh cells

doi: 10.1016/j.isci.2021.103566

Figure Lengend Snippet:

Article Snippet: Pure anti-human CCR5 , Miltenyi Biotec , Cat# 130-122-313; RRID:AB_2801894.

Techniques: Cell Analysis, Purification, Virus, Recombinant, Blocking Assay, Antibody Labeling, Transfection, Software

Journal: Science translational medicine

Article Title: Mixed-Effects Association of Single Cells Identifies an Expanded Effector CD4+ T Cell Subset in Rheumatoid Arthritis

doi: 10.1126/scitranslmed.aaq0305

Figure Lengend Snippet: RNA-seq characterization of CD627- HLA-DR+ (DR+27-) cells and 6 related CD4+ T cell populations: naive T cells (Tnaive), regulatory T cells (Treg), central memory t cells (TCM), and three populations of effector memory T cells, CD27+ HLA-DR- (DR-27+), CD27+ HLA-DR+ (DR+27+), and CD27- HLA-DR- (DR-27-). (A) PCA plot (top) and PC1 gene loadings (bottom) of 90 samples from the 7 CD4+ T cell populations. Cells were colored on the PCA plot according to known cell type. Normal confidence ellipses at 1 standard deviation were plotted for each cell type. The 300 most positive and 300 most negative PC1 gene loadings for each cell type were averaged and plotted in the heatmap. Genes relevant to the CD27- HLA-DR+ population were labeled. (B) Gene set enrichment analysis was performed on all genes, ranked on their PC1 loadings. Two significantly enriched gene sets: NK signature (GSE22886 NAIVE CD4 T CELL VS NK CELL DN) and effector memory t cell signature (GSE11057 NAIVE VS EFF MEMORY CD4 T CELL) are shown. (C) Distribution of log-scaled expression of six canonical Th1 genes: CCR5, CIITA, CXCR3, IFNG, TBX21 (Tbet), and TNF. Populations are ordered by PC1 loading values, with CD27- HLA-DR+ population highlighted in red. (D) Distribution of log-scaled gene expression of six canonical cytotoxic genes: GNLY, GZMA, GZMB, GMZK, NKG7, and PRF1. Populations are ordered by PC1 loading values, with the CD27- HLA-DR+ population highlighted in red. Reported p-values in (C) and (D) correspond to a linear model of gene expression against ordered cell type (as an ordinal variable), with p-values adjusted for multiple testing by the Benjamini Hochberg procedure. (E) Cytokine expression determined by intracellular cytokine staining of peripheral effector memory CD4+ T cells after in vitro stimulation with PMA/ionomycin. The percentage of cells positive for each stain is plotted for CD27+ HLA-DR- and CD27- HLA-DR+ subsets. Each dot represents a separate donor (n = 12; 6 RA patients and 6 controls, except for the quantification of Granyzme A and perforin where n = 6; 3 RA patients and 3 controls). Statistical significance was assessed using a Wilcoxon signed-rank test.

Article Snippet: The samples were then incubated for 30 minutes at 4C on a shaker rack with 1ul of the following eighteen CyTOF surface antibodies in a cocktail brought to a volume of 50ul/sample in CSB: Anti-Human CD49D (9F10)-141Pr (Fluidigm), Anti-Human CCR5 (CD195)(–P-6G4) - 144Nd (Fluidigm), Anti-Human CD4 (RPA-T4) −145Nd (Fluidigm), Anti-Human CD8a (RPA-T8) −146Nd (Fluidigm), Anti-Human CD45RO-147Sm (Brigham and Women’s Hospital CyTOF Core), Anti-Human CD28–148Nd (BWH CyTOF Core), Anti-Human CD25 (IL-2R- (2A3) - 149Sm (Fluidigm), Anti-Human PD1–151Eu (BWH CyTOF Core), Anti-Human CD62L (DREG-56)-153Eu (Fluidigm), Anti-Human CD3 (UCHT1) −154Sm (Fluidigm), Anti-Human CD27 (L128) −155Gd (Fluidigm), Anti-Human CD183[CXCR3](G025H7)-156Gd (Fluidigm), Anti-Human CCR7–170Er (BWH CyTOF Core), Anti-Human ICOS-160Gd (BWH CyTOF Core), Anti-Human CD38 (HIT2)-167Er (Fluidigm), Anti-Human CD154 (CD40L) (24–31)-168Er (Fluidigm), Anti-Human CXCR5[CD185](51505)-171Yb (Fluidigm), and Anti-Human HLA-DR (L243) −174Yb (Fluidigm).

Techniques: RNA Sequencing, Standard Deviation, Labeling, Expressing, Gene Expression, Staining, In Vitro

Journal: Cell Reports Medicine

Article Title: Dominant CD4 + T cell receptors remain stable throughout antiretroviral therapy-mediated immune restoration in people with HIV

doi: 10.1016/j.xcrm.2023.101268

Figure Lengend Snippet:

Article Snippet: CCR5 NP-6G4 171Yb , Standard BioTools , Cat# 3171017A.

Techniques: Recombinant, Antibody Labeling, Staining, Cell Isolation, Control, Mass Cytometry, Sequencing, Software

Journal: Cell reports

Article Title: Comprehensive Immunoprofiling of Pediatric Zika Reveals Key Role for Monocytes in the Acute Phase and No Effect of Prior Dengue Virus Infection

doi: 10.1016/j.celrep.2020.107569

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-human CCR5 (clone NP-6G4) , Fluidigm , Cat# 3156015A; RRID:AB_2661814.

Techniques: Recombinant, Sensitive Assay, Labeling, Luminex, Software, Multiplex Assay